Table 2

Body composition and plasma parameters

After early LF dietAfter HF diet challenge
Lard
(n = 7)SAF
(n = 7)LIN
(n = 7)Lard
(n = 7)SAF
(n = 9)LIN
(n = 7)
BW (g)22.7 ± 0.7422.8 ± 0.4723.5 ± 0.7435.4* ± 1.6036.3* ± 2.1336.4* ± 1.63
Liver (% of BW)4.43a ± 0.073.61b ± 0.103.61b ± 0.074.00 ± 0.194.17 ± 0.253.78 ± 0.22
pVAT (% of BW)2.19 ± 0.172.23 ± 0.082.34 ± 0.176.19 ± 0.345.91 ± 0.426.33 ± 0.29
iSCAT (% of BW)1.33a ± 0.071.02b ± 0.041.16c ± 0.062.74 ± 0.282.84 ± 0.312.82 ± 0.18
Fat mass (% of BW)13.9a ± 0.4614.8 a ± 0.9512.9 b ± 0.2733.3* ± 1.7333.4* ± 2.4334.3* ± 1.88
TGs (mg/mL)0.43a ± 0.040.27b ± 0.030.29b ± 0.040.27* ± 0.020.32 ± 0.030.28 ± 0.02
Cholesterol (mg/mL)1.30a ± 0.061.17b ± 0.091.07b ± 0.121.68* ± 0.121.71* ± 0.121.69* ± 0.05
Adiponectin (µg/mL)15.5a ± 2.1720.9b ± 3.0722.3b ± 2.5928.66a* ± 1.6931.75ab* ± 1.6135.54b* ± 2.16
Leptin (pg/mL)40.1 ± 26.616.1 ± 10.521.0 ± 10.1526.4* ± 129.1890.5* ± 156.1622.6* ± 141.3
Insulin (pg/mL)25.4a ± 15.4108.3b ± 36.0131.5b ± 62.7715.5* ± 168.3837.0* ± 151.5755.9* ± 119.2
Glucose (g/L)1.76 ± 0.061.70 ± 0.021.63 ± 0.102.07* ± 0.142.09* ± 0.111.97* ± 0.08
HOMA-IR20.0ab ± 2.6322.7a ± 3.4015.4bc ± 1.98
DI0–15/DG0–15−176a ± 2202,344b ± 6461,993b ± 504
DI0−0.002a ± 0.0050.056b ± 0.0140.060b ± 0.013
  • All parameters except glucose levels were measured from blood and tissue samples collected on the day of sacrifice from overnight-fasted animals. Glucose levels and calculations were determined from blood samples collected during OGTT experiments initiated with animals fasted for 6 h. BW, body weight; DI0 –15/DG0–15, insulin production to glucose load (µIU/mmol); DI0, oral disposition index (mmol−1) = (DI0–15/DG0–15)*1/fasting insulin; HOMA-IR, fasting glucose (mmol/L)*fasting insulin (µIU/L)/22.5; iSCAT, inguinal subcutaneous adipose tissue; pVAT, periepididymal adipose tissue. Results are expressed as the mean ± SEM (n = 7–9). Different superscript letters (a, b, c) indicate significant statistical differences between groups after early LF diet or after HF diet challenge at P < 0.05.

  • *Significant statistical differences between HF diet challenge and corresponding early LF diet means (P < 0.05).