TABLE 4

Effects of elevated fatty acid supply on ventricular function in perfused working hearts from control (db/+), type 2 (db/db), and type 1 (STZ-administered db/+) diabetic mice

db/+ (n = 6)
db/db (n = 8)
STZ (n = 7)
Low fatty acidsHigh fatty acidsLow fatty acidsHigh fatty acidsLow fatty acidsHigh fatty acids
Heart rate (bpm)334 ± 11320 ± 18295 ± 12281 ± 12279 ± 13281 ± 12
End-systolic pressure (mmHg)69 ± 270 ± 274 ± 376 ± 277 ± 477 ± 3
End-diastolic pressure (mmHg)7 ± 18 ± 19 ± 110 ± 18 ± 19 ± 1
dP/dtmax (mmHg/s)4,935 ± 1995,005 ± 2485,483 ± 3435,499 ± 2075,419 ± 2925,315 ± 385
dP/dtmin (mmHg/s)−4,352 ± 203−4,152 ± 257−4,682 ± 264−4,451 ± 254−4,943 ± 187−4,857 ± 296
Tau (ms)15 ± 115 ± 118 ± 320 ± 313 ± 114 ± 1
Cardiac output (ml/min)11.4 ± 0.710.8 ± 0.79.4 ± 0.39.4 ± 0.311.3 ± 1.211.5 ± 1.2
Coronary flow rate (ml/min)2.5 ± 0.42.6 ± 0.52.1 ± 0.12.2 ± 0.12.3 ± 0.22.4 ± 0.2
Emax3.4 ± 0.33.7 ± 0.66.6 ± 0.95.6 ± 0.9*3.6 ± 0.53.4 ± 0.4
PRSWi51.5 ± 3.745.6 ± 6.257.3 ± 3.248.3 ± 5.4*50.3 ± 2.546.9 ± 3.6
EDPVR · 10−26.5 ± 0.66.7 ± 0.99.2 ± 1.18.6 ± 1.15.3 ± 0.45.7 ± 0.6
  • Parameters of cardiac function were measured with low and high fatty acid concentrations (before and after the buffer replacement) in working hearts at 8 mmHg preload and 50 mmHg afterload. The relaxation constant Tau (glanz) is the regression of dP/dt versus pressure. The time-varying maximal ventricular elastance (Emax), preload recruitable stroke work index (PRSWi), and exponential fit of the relationship between pressure and volume at end diastole (EDPVR) were assessed from a family of pressure-volume loops created by a temporary preload occlusion.

  • *

    * P < 0.05 vs. low fatty acids.