TABLE 2

Respiration rates with different oxidative substrates for mitochondria isolated from control (db/+), type 2 (db/db), and type 1 (STZ-administered db/+) diabetic mice

Pyruvate
Malate/carnitine-l-palmitoyl
db/+db/dbSTZdb/+db/dbSTZ
State 216.9 ± 2.114.3 ± 1.215.2 ± 1.339.1 ± 2.737.8 ± 2.723.9 ± 4.5*
State 368.7 ± 11.053.1 ± 4.730.3 ± 4.0*103.7 ± 23.5157.9 ± 13.7*56.3 ± 13.7
State 413.2 ± 3.69.2 ± 2.78.3 ± 4.922.6 ± 6.423.3 ± 5.513.3 ± 3.9
  • Data are means ± SE. Mitochondrial respiration was measured using either 7 mmol/l pyruvate or 25 μmol/l palmitoyl-carnitine/5 mmol/l malate as substrates (n = 5–8). The mitochondrial respiration data are expressed in nmol O2 · min−1 · mg protein−1. Basal (state 2) respiration was measured in the absence of added ADP; rates of state 3 respiration were recorded after the addition of 300 μmol/l ADP, whereas state 4 respiration was recorded after complete phosphorylation of added ADP.

  • *

    * P < 0.05 vs. db/+.